Whole Genome Methylation Standard reference Genome-In-A-Bottle (GIAB) samples Hg001/HG002/HG005

Three standard GIAB reference samples HG001/HG002/HG005 were sequenced on a UG 100™ sequencer with a read-length of ~200bp (as recommended by DNA methylation conversion kits). The data was base-called with base-calling pipeline version APL4.6 and quality filtered by read quality (rq≤1). Reads were then trimmed for quality and adapters using Cutadapt and mapped to the human genome (hg38) using bwa-meth. Methylation calls were generated using MethylDackel.