Focus Area: Gene & Protein Expression
The UG 100™ sequencing platform enables high-depth, high-throughput RNA-sequencing allowing researchers to dive deeper into the transcriptome for detection of novel transcripts and isoforms.
What is it?
RNA sequencing
Why it matters
RNA sequencing illuminates the dynamic nature of life; the way that cells react to stimuli through the differential expression and regulation of RNA.
Whole transcriptome sequencing is a useful tool for measuring gene expression levels without the biases of targeted approaches, while higher depth RNA-seq provides the opportunity to find novel isoforms and splice variants. The UG 100 offers scientists benefits for RNA-seq studies including high-quality RNA-seq data that compares favorably to gold standard data from the SEQC Consortium, cost effective ultra-deep sequencing allowing for the detection of lowly expressed genes, and 300bp contiguous reads for enhanced isoform detection.
How Ultima transforms the field
Detect rare events
as low as one-in-a-million
Ultima puts a new spin on single-cell and spatial Analysis through transformative economics on the UG 100™ Sequencer to enable more cells, more samples, and more expansive experiments (treatments, timepoints, perturbations).
The transformative economics of Ultima genomics mostly natural sequencing mean that you can conduct deep RNA sequencing - over 400M reads per sample - and to validate findings and confirm isoform abundance.
Read our deep RNA-seq publication
Transcriptome profiling on UG 100 is highly concordant with other methods of differential gene expression detection
Learn how Pertub-Seq can reconstruct pathway signatures
Figure reference: Correlation of differential expression between UHRR and HBRR samples from RNA-seq libraries sequenced on the UG 100 vs TaqMan (left) or sequencing reference data (right) from the SEQC Consortium for the top 843 expressed genes (Su et al 2014)