Genome-scale perturb-seq in primary human CD4+ T cells maps context-specific regulators of T cell programs and human immune traits

Genome wide Perturbation of all 12,748 genes expressed in CD4 T cells across 4 donors using CRISPRi and 10x Flex Gene Expression analysis which lead to 11,527 transcriptome impacts being successfully estimated. 67% of perturbed gene showed significant Differential Expression on at least 3 other genes and discovered over 2M significant regulator to gene trans-effects.