Genome-scale perturb-seq in primary human CD4+ T cells maps context-specific regulators of T cell programs and human immune traits

In this BioRxiv publication from Zhu, R. et al (Gladstone Institute, UCSF), genome-wide perturbation of all 12,748 expressed genes in CD4 T cells was performed across 4 donors using CRISPRi and 10x Flex Gene Expression analysis.