Secondary Analysis Pipelines for Proteomics Analysis

Secondary Analysis Steps for Olink Explore HT Sequencing Data

Trimming
The Ultima Trimmer software tool divides each read into segments, trimming Ultima adapter sequence and remaining conserved sequence, while keeping the Ultima barcode, the Olink forward and reverse barcodes for protein identification, as well as the Olink plate index sequence.
Tagging
Reads are tagged (Ultima barcode, Olink barcodes, and Olink index).
Demultiplex
Reads are sorted, per sample, into folders based on Ultima barcode and Olink index.
File Output
Read data is output as a CRAM file as well as an Olink counts file (hist.csv) ready for input into Olink's NPX™ analysis software.